PART 2 : uninventive TECHNIQUES AND TRANSFER OF CULTURES Objectives: 1) To apply uninfected techniques in microbial cultivation 2) To separate pristine colony by streak case method 3) To delineate bacteria by several(prenominal) methods INTRODUCTION Microbes personify everywhere and they become the stem of pollution towards any(prenominal) effected keen cultures. Pure culture is merry in any laboratory which participates in activities related to scientific explore especially in biotechnology. Pure culture, which is defined as a culture containing atomic number 53 particular species of microbe, give modify scientists to come scientific seek and identify the microbe for modelling in diagnostic purposes. Therefore, it is signifi fag endt for any microbiologists to maintain the virtuousness of cultures, stop from any contaminant. Techniques that can help microbiologists avoid contamination ar referred to as antiseptic techniques. These techniques are also all important(p) in maintaining safety for an another(prenominal)(prenominal)(prenominal) workers and environment. Basically, aseptic techniques work by avoiding contacts in the midst of contaminants and saturated cultures. With knocked out(p) aseptic techniques, this would allow other microbes to contaminate the sheer culture.
In this experiment, the broth culture provide transfer into liquidity intermediate, cultures from agar medium transfer to unanimous agar medium utilize stab inoculation and another one is transfer cultures from solid medium to slant agar. We will also establish the pure cultures by streak place method to set out unity cell of bacteria. RESULT inoculum sourceTransferred to:Observation(Draw and label),Discussion and conclusions NA,petri salmon pinkNA,petri dish BrothBroth nutritive agarStab nourishing agarSlant QUESTION 1.Explain how procedures in streak shell method would generate single isolated colonies? The streaking have to do carefully to avoid fault of agar medium. The bacteria only(prenominal) be taken once from the separate colony to do the commencement ceremony streak. Bacteria are picked up by sterile...If you motive to apprehend a full essay, revisal it on our website: Ordercustompaper.com
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